Malaysian Journal of Mathematical Sciences, February 2016, Vol. 10(S)
Special Issue: The 3rd International Conference on Mathematical Applications in Engineering 2014 (ICMAE'14)
Simulation of Absorption Spectrum of Photosynthetic Pigments of Chlorella Vulgaris B Algae
Mahmoud, G. S.,T. Hj. Hassan, Othman, R., and Messikh, A.
Corresponding Email: [email protected]
Received date: -
Accepted date: -
Abstract:
Absorption spectrum of Chlorella vulgaris B algae was reconstructed by simulated the absorption spectrum of the mixture under examination by a linear combination of the absorption spectra of all its constituents. A C30 reversed high performance liquid chromatography equipped with photodiode array detector (HPLC-PAD) and absorption spectrophotometer were used to separate and identify photosynthetic pigments of Chlorella vulgaris B. Spectrum-reconstruction method (SRCM) was used to calculate concentrations of total carotenoids, chlorophylls a and b. Absorption spectrum of the total pigments extracted of Chlorella vulgaris B. was reconstructed from the sum of the spectrum of individual extracted pigments taken from (HPLC-PAD), and compared using normalization with absorption spectrum of the total pigments recorded by spectrophotometer. A total of seven Pigments were separated and identified.
Keywords: Chlorella Vulgaris, Pigment Composition, Multi-Component Analysis, HPLC, Absorption Spectrum
